Lithium Hydroxide → HCID 42633276, compound

Participants

Reaction Steps

2-[5-({[(5-Methoxy[1,3]thiazolo[5,4-b]pyridin-2-yl)carbamoyl]amino}sulfonyl)-3-methyl-2-thienyl]ethyl acetate (235 mg, 0.5 mmol, 1 equiv.) was saponified by addition of 1N LiOH (1 mL, 1 mmol, 2 equiv.) for 60 min. The solution was purified by chromatography on a HPLC 75×30 mm RP18 5 μm column, with A=0.1% HCOOH and B=MeCN and with a gradient of 20% to 70% B in 10 min. The corresponding fractions were lyophilized to give 5-(2-hydroxyethyl)-N-[(5-methoxy[1,3]thiazolo[5,4-b]pyridin-2-yl)carbamoyl]-4-methylthiophene-2-sulfonamide, 47 mg, m/e 427.0 (MH−).

Provenance & Evidence