4-Aminopyridine → HCID 21104302, compound

Participants

Reaction Steps

1-Butyl-4-chloro-6-(2-fluoro-phenyl)-3-methyl-1H-pyrazolo[3,4-d]pyrimidine (100 mg, 0.31 mmole) and 4-aminopyridine (58 mg, 0.626 mmole) were combined in ethyleneglycol dimethoxy ether and heated to reflux for 4 hours. The reaction mixture was cooled and the product was isolated by filtration, washed with minimum cold solvent and dried to give 132 mg of product. 50 mg of this material was subjected to HPLC purification on reversed phase C18 column, eluting with gradient of water/acetonitrile/0.1% trifluoroacetic acid.

Provenance & Evidence