HuagongsheHuagongshe Chemistry Print Report · For research reference only
Sodium Chloride → Leukotriene B4
Participants
| Role | Name | HCID | Charge |
|---|---|---|---|
| Reactant | Sodium Chloride | HCID 5234 | — |
| Reactant | Ethylenediaminetetraacetic Acid | HCID 6049 | — |
| Reactant | Leukotriene A4 | HCID 5280383 | — |
| Reactant | methyl 4-[(2S,3S)-3-[(1E,3E,5Z,8Z)-tetradeca-1,3,5,8-tetraenyl]oxiran-2-yl]butanoate | HCID 6478221 | — |
| Solvent | Potassium Phosphate, Tribasic | HCID 62657 | — |
| Product | Leukotriene B4 | HCID 5280492 | — |
Reaction Steps
The LTA4 substrate was freshly prepared by alkaline hydrolysis of LTA4 methyl ester (Cayman Chemical Co., USA). After incubating for 10 minutes in the presence of LTA4 (1 μM in 50 mM Tris-HCl, 0.15 M NaCl, 0.5% BSA, pH 7.4), the reaction is stopped by diluting 1/20 in 0.1 M potassium phosphate buffer containing 1.5 mM NaN2, 0.4 M NaCl, 1 mM EDTA, 0.1% BSA, pH 7.4, −4° C.
Provenance & Evidence
| DOI | 10.6084/m9.figshare.5104873.v1 |
|---|---|
| Patent number | US07094773B2 |
| Source dataset | uspto-grants-2006_08 |
| ORD ID | ord-2c4f74d36a3e4147b27538559e142388 |
| Source note | uspto-grants-2006_08 |