反应详情

This procedure was carried out as described previously in Example 19, using Compound 17 (500 mg, 1.52 mmol) and N,N,N′-trimethylethylenediamine (988 μL, 7.6 mmol). The dye was obtained as a red solid (260 mg) after Biotage purification using a gradient of methanol in chloroform. Abs (max, PBS pH 7.4)=530 nm; Em=646 nm. The structure of Compound 41 is given below: