Sodium borohydride → 2-(3-(Hydroxymethyl)-4-(1-methyl-5-(5-methyl-4,5,6,7-tetrahydro-pyrazolo[1,5-a]pyrazin-2-ylamino)-6-oxo-1,6-dihydropyridin-3-yl)pyridin-2-yl)-3,4,6,7,8,9-hexahydropyrido[3,4-b]indolizin-1(2H)-one

HRID 1021740Notes

Reaction equation

HRID 1021740 reaction equation
[CH:1]([C:3]1[C:4]([N:28]2[CH2:40][CH2:39][C:38]3[N:37]4[C:32]([CH2:33][CH2:34][CH2:35][CH2:36]4)=[CH:31][C:30]=3[C:29]2=[O:41])=[N:5][CH:6]=[CH:7][C:8]=1[C:9]1[CH:14]=[C:13]([NH:15][C:16]2[CH:25]=[C:19]3[CH2:20][N:21]([CH3:24])[CH2:22][CH2:23][N:18]3[N:17]=2)[C:12](=[O:26])[N:11]([CH3:27])[CH:10]=1)=[O:2].[BH4-].[Na+]>CO>[OH:2][CH2:1][C:3]1[C:4]([N:28]2[CH2:40][CH2:39][C:38]3[N:37]4[C:32]([CH2:33][CH2:34][CH2:35][CH2:36]4)=[CH:31][C:30]=3[C:29]2=[O:41])=[N:5][CH:6]=[CH:7][C:8]=1[C:9]1[CH:14]=[C:13]([NH:15][C:16]2[CH:25]=[C:19]3[CH2:20][N:21]([CH3:24])[CH2:22][CH2:23][N:18]3[N:17]=2)[C:12](=[O:26])[N:11]([CH3:27])[CH:10]=1

Participants

4

Reactant 2

HCID 4311764Sodium borohydrideHCID 866842592-(3-Formyl-4-(1-methyl-5-(5-methyl-4,5,6,7-tetrahydropyrazolo[1,5-a]pyrazin-2-ylamino)-6-oxo-1,6-dihydropyridin-3-yl)pyridin-2-yl)-3,4,6,7,8,9-hexahydropyrido[3,4-b]indolizin-1(2H)-one

Product 1

HCID 715571052-(3-(Hydroxymethyl)-4-(1-methyl-5-(5-methyl-4,5,6,7-tetrahydro-pyrazolo[1,5-a]pyrazin-2-ylamino)-6-oxo-1,6-dihydropyridin-3-yl)pyridin-2-yl)-3,4,6,7,8,9-hexahydropyrido[3,4-b]indolizin-1(2H)-oneYield 60.1%

Reagents, catalysts & solvents 1

HCID 887MethanolSolvent

Conditions

Temperature
25 °C

Procedure

A mixture of 147b (100 mg, 0.18 mmol), NaBH4 (21 mg, 0.54), and CH3OH (10 mL) was stirred at 25° C. for 1 h. The mixture was extracted with CH2Cl2 (10 mL×2). The combined CH2Cl2 extract was concentrated under reduced pressure. The residue was purified with reverse-phase prep-HPLC to afford 147 (60 mg, 60%). LCMS: [M+H]+ 555. 1H NMR (500 MHz, DMSO) δ 8.45 (d, J=5.0, 1H), 8.19 (s, 1H), 8.06 (d, J=5.0, 1H), 7.41 (d, J=2.0, 1H), 7.29 (d, J=5.0, 1H), 6.04 (s, 1H), 5.88 (s, 1H), 4.92 (s, 1H), 4.33-4.42 (m, 2H), 4.11-4.16 (m, 1H), 3.91-3.96 (m, 3H), 3.77-3.82 (m, 2H), 3.57 (s, 3H), 3.45-3.48 (m, 2H), 2.91-3.01 (m, 2H), 2.71-2.79 (m, 4H), 2.35 (s, 3H), 1.90-1.92 (m, 2H), 1.71-1.79 (m, 2H)

Workup

  1. extractionThe mixture was extracted with CH2Cl2 (10 mL×2)
  2. extractionThe combined CH2Cl2 extract
  3. concentrationwas concentrated under reduced pressure
  4. customThe residue was purified with reverse-phase prep-HPLC

Sources & evidence

ORD record
ord-927f970210a94fcfa1a015d59d4045f8
Source dataset
uspto-grants-2014_05
Patent number
US08716274B2

Reaction notes

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