Sodium borohydride → 2-[3-(hydroxymethyl)-4-[1-methyl-5-[(5-methyl-6,7-dihydro-4H-pyrazolo[1,5-a]pyrazin-2-yl)amino]-6-oxo-3-pyridyl]-2-pyridyl]-6,7,8,9-tetrahydropyrazino[1,2-a]indol-1-one

HRID 1021896Notes

Reaction equation

HRID 1021896 reaction equation
[CH:1]([C:3]1[C:4]([N:28]2[CH:40]=[CH:39][N:31]3[C:32]4[CH2:33][CH2:34][CH2:35][CH2:36][C:37]=4[CH:38]=[C:30]3[C:29]2=[O:41])=[N:5][CH:6]=[CH:7][C:8]=1[C:9]1[CH:14]=[C:13]([NH:15][C:16]2[CH:25]=[C:19]3[CH2:20][N:21]([CH3:24])[CH2:22][CH2:23][N:18]3[N:17]=2)[C:12](=[O:26])[N:11]([CH3:27])[CH:10]=1)=[O:2].[BH4-].[Na+]>CO>[OH:2][CH2:1][C:3]1[C:4]([N:28]2[CH:40]=[CH:39][N:31]3[C:32]4[CH2:33][CH2:34][CH2:35][CH2:36][C:37]=4[CH:38]=[C:30]3[C:29]2=[O:41])=[N:5][CH:6]=[CH:7][C:8]=1[C:9]1[CH:14]=[C:13]([NH:15][C:16]2[CH:25]=[C:19]3[CH2:20][N:21]([CH3:24])[CH2:22][CH2:23][N:18]3[N:17]=2)[C:12](=[O:26])[N:11]([CH3:27])[CH:10]=1

Participants

4

Reactant 2

HCID 4311764Sodium borohydrideHCID 866843592-(3-(Formyl)-4-(1-methyl-5-(5-methyl-4,5,6,7-tetrahydropyrazolo[1,5-a]pyrazin-2-ylamino)-6-oxo-1,6-dihydropyridin-3-yl)pyridin-2-yl)-6,7,8,9-tetrahydro-pyrazino[1,2-a]indol-1(2H)-one

Product 1

HCID 895396192-[3-(hydroxymethyl)-4-[1-methyl-5-[(5-methyl-6,7-dihydro-4H-pyrazolo[1,5-a]pyrazin-2-yl)amino]-6-oxo-3-pyridyl]-2-pyridyl]-6,7,8,9-tetrahydropyrazino[1,2-a]indol-1-oneYield 58%

Reagents, catalysts & solvents 1

HCID 887MethanolSolvent

Procedure

To a solution of 195g (120 mg, 0.22 mmol) in methanol (5 mL) at 0° C. was added sodium borohydride (25 mg, 0.66 mmol). The reaction mixture was stirred for 30 minutes. It was then quenched with water (1.0 mL) and concentrated under reduced pressure. The residue was purified by reverse-phase prep-HPLC to afford 195 (70 mg, 58%). MS-ESI: [M+H]+ 553.3. 1H NMR (500 MHz, CDCl3) δ 8.56 (d, J=5.0 Hz, 1H), 7.99 (d, J=2.0 Hz, 1H), 7.75 (d, J=2.0 Hz, 1H), 7.49 (d, J=4.5 Hz, 1H), 7.43 (s, 1H), 7.07 (s, 1H), 6.97 (d, J=6.0 Hz, 1H), 6.67 (d, J=6.0 Hz, 1H), 5.70 (s, 1H), 5.08-5.06 (m, 1H), 4.51-4.49 (m, 1H), 4.36-4.34 (m, 1H), 4.14-4.05 (m, 2H), 3.72 (s, 3H), 3.62-3.60 (m, 2H), 2.91-2.89 (m, 2H), 2.75-2.70 (m, 4H), 2.49 (s, 3H), 1.97-1.95 (m, 2H), 1.86-1.84 (m, 2H).

Workup

  1. customIt was then quenched with water (1.0 mL)
  2. concentrationconcentrated under reduced pressure
  3. customThe residue was purified by reverse-phase prep-HPLC

Sources & evidence

ORD record
ord-39a8625bedab4b68bc06c6ee69bee0a8
Source dataset
uspto-grants-2014_05
Patent number
US08716274B2

Reaction notes

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