反应详情

To a mixture of 1-(tert-butoxycarbonyl)-2-methylproline (425 mg), triethylamine (0.425 mL) and tetrahydrofuran (10 mL) was added 2-methylpropyl chlorocarbonate (0.2 mL) with stirring at room temperature. After 1 hr, 3-amino-5-bromothiophene-2-carboxamide (337 mg) produced in Example 1, step D, was added, and the mixture was stirred at 60° C. overnight. Thereafter, the mixture was stirred in a microwave reactor at 120° C. for 4 hr. The reaction mixture was allowed to cool to room temperature, and poured into saturated aqueous sodium hydrogen carbonate. The mixture was extracted with ethyl acetate, and the extract was dried over anhydrous magnesium sulfate. Insoluble material was removed by filtration, and the filtrate was concentrated under reduced pressure. The residue was dissolved in methanol (5 mL), 2M aqueous sodium hydroxide solution (2.04 mL) was added, and the mixture was stirred at 60° C. for 1 hr and at 80° C. for 1 hr. Ethanol (4 mL) was added to the reaction mixture, and the mixture was stirred at 100° C. for 4 hr. The reaction mixture was allowed to cool to room temperature, and poured into saturated aqueous sodium hydrogen carbonate, and the mixture was extracted with ethyl acetate/tetrahydrofuran mixture. The extract was dried over anhydrous magnesium sulfate. Insoluble material was removed by filtration, and the filtrate was concentrated under reduced pressure. The residue was purified by basic silica gel column chromatography (ethyl acetate/hexane and methanol/ethyl acetate) to give the title compound (109 mg) as a yellow solid.

1. stirringthe mixture was stirred at 60° C. overnight
2. stirringThereafter, the mixture was stirred in a microwave reactor at 120° C. for 4 hr
3. temperatureto cool to room temperature
4. extractionThe mixture was extracted with ethyl acetate
5. dry with materialthe extract was dried over anhydrous magnesium sulfate
6. customInsoluble material was removed by filtration
7. concentrationthe filtrate was concentrated under reduced pressure
8. dissolutionThe residue was dissolved in methanol (5 mL)
9. addition2M aqueous sodium hydroxide solution (2.04 mL) was added
10. stirringthe mixture was stirred at 60° C. for 1 hr and at 80° C. for 1 hr
11. additionEthanol (4 mL) was added to the reaction mixture
12. stirringthe mixture was stirred at 100° C. for 4 hr
13. temperatureto cool to room temperature
14. additionpoured into saturated aqueous sodium hydrogen carbonate
15. extractionthe mixture was extracted with ethyl acetate/tetrahydrofuran mixture
16. dry with materialThe extract was dried over anhydrous magnesium sulfate
17. customInsoluble material was removed by filtration
18. concentrationthe filtrate was concentrated under reduced pressure
19. customThe residue was purified by basic silica gel column chromatography (ethyl acetate/hexane and methanol/ethyl acetate)