Ammonium Chloride → 6-[4-(6-amino-pyridin-3-yl)-phenyl]-5-(4-chloro-phenyl)-1-[3-(1H-tetrazol-5-yl)-phenyl]-1,5-dihydro-pyrazolo[3,4-d]pyrimidin-4-one

Participants

Reaction Conditions

Reaction Steps

To a solution of 3-[6-[4-(5-amino-pyridin-2-yl)-phenyl]-5-(4-chloro-phenyl)-4-oxo-4,5-dihydro-pyrazolo[3,4-d]pyrimidin-1-yl]-benzonitrile (prepared as described in example 28, 0.375 g, 0.726 mmol) in N,N-dimethylformamide (7 mL), sodium azide (0.236 g, 3.63 mmol), ammonium chloride (0.19 g, 3.63 mmol) are added. The reaction mixture is then heated at 90° C. for 14 h. The reaction mixture is concentrated to a dry residue. It is purified by column chromatography to afford 6-[4-(6-amino-pyridin-3-yl)-phenyl]-5-(4-chloro-phenyl)-1-[3-(1H-tetrazol-5-yl)-phenyl]-1,5-dihydro-pyrazolo[3,4-d]pyrimidin-4-one. 1H NMR (DMSO-d6, 400 MHz) δ 8.81 (s, 1H), 8.57 (s, 1H), 8.50 (br, 1H), 8.38 (m, 1H), 8.27 (m, 1H), 8.07 (d, 1H), 7.79 (m, 2H), 7.55 (m, 2H), 7.39-7.46 (m, 5H), 6.59 (d, 1H), 6.54 (br, 1H); LC-MS calculated for C29H19ClN10O (M+H+) 559.1, found 559.1.

Provenance & Evidence