Glycerol → L-Lysine

The glycerol stocks of the Escherichia coli WC196ΔcadAΔldcC/pCABD2 strain were thawed, 100 μL of each was uniformly applied to an L-plate containing 25 mg/L of streptomycin, and culture was performed at 37° C. for 20 hours. The cells in an amount of about ⅛ of the cells obtained on one plate were inoculated in 20 mL of the fermentation medium described below containing 25 mg/L of streptomycin in a Sakaguchi flask, and cultured at 37° C. for 16 hours or 20 hours on a reciprocally shaking culture apparatus. Each of the samples derived from the alga obtained in of Example 7 (2) was suspended in distilled water, Tween 80 was added to the suspension at a concentration of 1%, and the mixture was adjusted to pH 7.0 with 3 N KOH, autoclaved at 120° C. for 20 minutes, and used as the carbon source solution. The medium composition used for the culture is shown below. The hydrolysate of the fat-and-oil fraction derived from the alga, or the water-soluble fraction derived from the alga was used as the carbon source. The totals of the measured aliphatic acid concentration and glycerol concentration of the carbon sources are indicated.

1. customThe cells in an amount of about ⅛ of the cells obtained on one plate
2. waitcultured at 37° C. for 16 hours or 20 hours
3. waitautoclaved at 120° C. for 20 minutes
4. concentrationThe totals of the measured aliphatic acid concentration and glycerol concentration of the carbon sources

ORD record

ord-118570957a02402a8a251a6eaef54f37

Source dataset

uspto-grants-2014_05

DOI

10.6084/m9.figshare.5104873.v1

Patent number

US08728772B2