反应详情

A mixture of 200 mg of methyl 4-{2-[4-({[2-({3-[(3-tert-butoxy-3-oxopropyl)(methyl)sulfamoyl]benzoyl}amino)-4,5,6,7-tetrahydro-1-benzothiophen-3-yl]carbonyl}amino)phenyl]ethyl}benzoate, 2.0 mL of trifluoroacetic acid, and 2.0 mL of dichloromethane was stirred at room temperature for 4 hours. The reaction mixture was concentrated under reduced pressure, and to the obtained crude product were added 0.50 mL of a 5.0 M aqueous sodium hydroxide solution and 2.0 mL of ethanol, followed by heating and refluxing overnight. The reaction mixture was concentrated under reduced pressure and to the obtained residue were added water and citric acid (500 mg), followed by extraction with dichloromethane. The organic layer was concentrated under reduced pressure and the obtained residue was purified by silica gel column chromatography (chloroform-methanol). The crude product was washed with ethyl acetate to obtain 135 mg of 4-{2-[4-({[2-({3-[(2-carboxyethyl)(methyl)sulfamoyl]benzoyl}amino)-4,5,6,7-tetrahydro-1-benzothiophen-3-yl]carbonyl}amino)phenyl]ethyl}benzoic acid as a colorless crystal.

1. concentrationThe reaction mixture was concentrated under reduced pressure
2. customto the obtained crude product
3. temperatureby heating
4. temperaturerefluxing overnight
5. concentrationThe reaction mixture was concentrated under reduced pressure and to the obtained residue
6. additionwere added water and citric acid (500 mg)
7. extractionfollowed by extraction with dichloromethane
8. concentrationThe organic layer was concentrated under reduced pressure
9. customthe obtained residue was purified by silica gel column chromatography (chloroform-methanol)
10. washThe crude product was washed with ethyl acetate