HuagongsheHuagongshe Chemistry Print Report · For research reference only
Chloramphenicol → L-Phenylalanine
Participants
| Role | Name | HCID | Charge |
|---|---|---|---|
| Reactant | Chloramphenicol | HCID 5959 | — |
| Reactant | Kanamycin | HCID 6032 | — |
| Reactant | L-Tyrosine | HCID 6057 | — |
| Reactant | Ammonium Chloride | HCID 25517 | — |
| Reactant | (2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal | HCID 107526 | — |
| Solvent | Mops | HCID 70807 | — |
| Product | L-Phenylalanine | HCID 6140 | — |
Reaction Steps
L-tyrosine production experiments were performed at 37° C. with 225 rpm orbital shaking in 50 ml MOPS minimal medium (Teknova) (Neidhardt, Bloch et al. 1974) cultures supplemented with 5 g/l glucose and an additional 4 g/l NH4Cl. All liquid cultivations were conducted in at least triplicates. When appropriate, antibiotics were added in the following concentrations: 34 μg/ml chloramphenicol for maintenance of pHACM-derived plasmids and 20 μg/ml kanamycin for maintenance of pZE-derived plasmids. L-phenylalanine auxotrophic (ΔpheA) cultures were additionally provided with L-phenylalanine (Sigma) at a concentration of 0.35 mM.
Provenance & Evidence
| DOI | 10.6084/m9.figshare.5104873.v1 |
|---|---|
| Patent number | US08735132B2 |
| Source dataset | uspto-grants-2014_05 |
| ORD ID | ord-afa5e19ef9954e55a90885585ba46ecf |
| Source note | uspto-grants-2014_05 |