Ethyl beta-[[3-[[[4(aminoiminomethyl)phenyl]-amino]thiocarbonyl]aminopropanoyl]amino]propanoate → beta-[[3-[[[4(Aminoiminomethyl)phenyl]amino]-thiocarbonyl]aminopropanoyl]amino]propanoic acid

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Reaction Steps

Ethyl β-[[3-[[[4(aminoiminomethyl)phenyl]amino]thiocarbonyl]aminopropanoyl]amino]propanoate (65 mg; 0.18 mmoles) was treated in 1N lithium hydroxide/methanol (20 ml; 1:1) for 10 min. Methanol was evaporated and the pH of the remaining mixture was adjusted to 5 with 50% acetic acid. The mixture was then purified by HPLC using a linear gradient of acetonitrile/water/0.05% trifluoro-acetic acid (0-20% acetonitrile in 30 min). Yield: 51 mg (85%). FAB-MS: MH+ =338.0.

Provenance & Evidence