HuagongsheHuagongshe Chemistry Print Report · For research reference only
6-Carboxyfluorescein → 2-(6-Hydroxy-3-oxo-3H-xanthen-9-yl)benzoic acid
Participants
| Role | Name | HCID | Charge |
|---|---|---|---|
| Reactant | 6-Carboxyfluorescein | HCID 76806 | — |
| Reactant | Diisopropylethylamine | HCID 81531 | — |
| Reactant | Methanaminium, N-((dimethylamino)(3H-1,2,3-triazolo(4,5-b)pyridin-3-yloxy)methylene)-N-methyl-, hexafluorophosphate(1-) (1:1) | HCID 9886157 | — |
| Solvent | N-methyl-2-pyrrolidone | HCID 13387 | — |
| Product | 2-(6-Hydroxy-3-oxo-3H-xanthen-9-yl)benzoic acid | HCID 3383 | — |
Reaction Steps
5(6)-carboxy fluorescein is dissolved in NMP to a concentration of 0.2 M. Activation is performed with 0.9 eq. HATU and 1 eq. DIPEA for 2 min before coupling for at least 2×20 min or until the Kaiser test is negative.
Provenance & Evidence
| DOI | 10.6084/m9.figshare.5104873.v1 |
|---|---|
| Patent number | US08546309B2 |
| Source dataset | uspto-grants-2013_10 |
| ORD ID | ord-c0da6965385c4e94892ebde574e24b51 |
| Source note | uspto-grants-2013_10 |