反应详情

To a cold (0° C.) solution of (S)-2-{[(R)-7-(3,5-dichloro-phenyl)-5-(4′-fluoro-biphenyl-4-ylmethyl)-5-methyl-6-oxo-6,7-dihydro-5H-imidazo[1,2-a]imidazole-3-carbonyl]-amino}-propionic acid (0.12 g, 0.21 mmol) in DMF (1 mL) was added (R)-3-(1H-tetrazol-5-yl)-piperidine trifluoro-acetic acid salt (0.055 g, 0.21 mmol), HATU (0.093 mg, 0.25 mmol) and Et3N (0.059 mL, 0.41 mmol). The reaction was allowed to stir for 10 min and then HOAt (0.056 g, 0.41 mmol) was added. The mixture was allowed to warm to room temperature overnight. The crude reaction mixture was purified by reverse phase HPLC to give 35 mg of the title compound as a white solid, m/z 716.6 [M]+.

1. customThe crude reaction mixture
2. customwas purified by reverse phase HPLC