Sodium Bicarbonate → HCID 45110295, compound

Participants

Reaction Steps

1-(2-Amino-3-methyl-butyryl)-pyrrolidine-2-carboxylic acid [3-oxo-1-(2,4,5-trifluoro-benzyl)-3-(3-trifluoromethyl-5,6-dihydro-8H-[1,2,4]triazolo[4,3-a]pyrazin-7-yl)-propyl]-amide Hydrochloride (Compound of formula 9) (520 mg, 0.8 mmole) was dissolve in water 95 ml). Then saturated sodium bicarbonate solution (6 ml) was added to this solution till pH become basic (pH˜10) for breaking of Hydrochloride salt. Then compound was extracted with EtoAC (3×75 ml). Organic extracts was dried over Na2SO4 and concentrated under vacuum to yield 1-(2-Amino-3-methyl-butyryl)-pyrrolidine-2-carboxylic acid [3-oxo-1-(2,4,5-trifluoro-benzyl)-3-(3-trifluoromethyl-5,6-dihydro-8H-[1,2,4]triazolo[4,3-a]pyrazin-7-yl)-propyl]-amide free base (Compound of formula 10). (Yield=480 mg=98%).

Provenance & Evidence