反应详情

A solution of 6-[(2,2-diphenylethyl)amino]-9H-purine-2-carbonitrile (Preparation 6) (5.0 g, 14.7 mmol) and sodium methoxide (4.0 g, 74.1 mmol) in methanol (300 ml) was heated under reflux for 24 hours. Further sodium methoxide (2.0 g, 37 mmol) and methanol (100 ml) was added and heating continued for a further 24 hours. The reaction mixture was allowed to cool and the solvent removed under reduced pressure. The residue was dissolved in tetrahydrofuran (THF) (375 ml), 2N aqueous hydrochloric acid (125 ml) added and the mixture stirred at room temperature for 24 hours. The THF was removed under reduced pressure and the suspension basified to pH 7 with saturated aqueous sodium bicarbonate solution. Ethyl acetate (100 ml) was added and the white solid consisting mainly of the desired product filtered, washed with a little water and ethyl acetate and dried. Purification by column chromatography on silica gel eluting with a gradient system of dichloromethane:methanol (90:10, by volume) gradually changing to dichloromethane:methanol (75:25, by volume) afforded the title compound as a white solid, 1.25 g (25%). Evaporation of the ethyl acetate filtrate provided 2.6 g of the starting material.

1. temperatureunder reflux for 24 hours
2. temperatureheating
3. temperatureto cool
4. customthe solvent removed under reduced pressure
5. dissolutionThe residue was dissolved in tetrahydrofuran (THF) (375 ml)
6. addition2N aqueous hydrochloric acid (125 ml) added
7. customThe THF was removed under reduced pressure
8. additionEthyl acetate (100 ml) was added
9. filtrationfiltered
10. washwashed with a little water and ethyl acetate
11. customdried
12. customPurification by column chromatography on silica gel eluting with a gradient system of dichloromethane:methanol (90:10, by volume)