反应详情

温度

50 °C

(S)-Methyl 3-(4-amino-6-((1-(5-methyl-4-oxo-3-phenyl-3,4-dihydropyrrolo[2,1-f][1,2,4]triazin-2-yl)ethyl)amino)pyrimidin-5-yl)-5-hydroxybenzoate (35 mg, 0.07 mmol) was dissolved in 5 mL tetrahydrofuran. Lithium hydroxide in 5 mL water was added and the mixture was stirred at 50° C. for 4 h. The solvent was evaporated and the residue was re-dissolved in dichloromethane. The aqueous phase was acidified with 2N hydrochloric acid. The aqueous was further extracted with dichloromethane and was washed with water and brine, dried over sodium sulphate, filtered and evaporated under reduced pressure. The residue was purified by reverse phase using SP1 ® Purification System to obtain 25 mg (72% yield) of the title compound as a white solid. Purity 97%.

1. customThe solvent was evaporated
2. dissolutionthe residue was re-dissolved in dichloromethane
3. extractionThe aqueous was further extracted with dichloromethane
4. washwas washed with water and brine
5. dry with materialdried over sodium sulphate
6. filtrationfiltered
7. customevaporated under reduced pressure
8. customThe residue was purified by reverse phase
9. custom® Purification System