反应详情

温度

120 °C

To a solution of (S)-2-(1-aminoethyl)-5-(6-(4-isopropylpiperazin-1-yl)-6-oxohex-1-yn-1-yl)-3-phenylpyrrolo[2,1-f][1,2,4]triazin-4(3H)-one (120 mg, purity 10%, 0.03 mmol) and 4-amino-6-chloropyrimidine-5-carbonitrile (6 mg, 0.04 mmol) in tert-butanol (3 ml) was added DIEA (44 μl, 0.25 mmol) and the reaction mixture was stirred at 120° C. for 2 days. The solvent was removed under reduced pressure. Ethyl acetate was added and the organic phase was washed with water and brine, dried over magnesium sulphate, filtered and the solvents were removed under reduced pressure. The product was purified by flash chromatography using SP1® Purification System (0% to 100% hexane/AcOEt, 0-8% AcOEt/MeOH) to give 4 mg (25% yield) of the title compound as a white solid. Purity 96%.

1. customThe solvent was removed under reduced pressure
2. additionEthyl acetate was added
3. washthe organic phase was washed with water and brine
4. dry with materialdried over magnesium sulphate
5. filtrationfiltered
6. customthe solvents were removed under reduced pressure
7. customThe product was purified by flash chromatography
8. customSP1® Purification System (0% to 100% hexane/AcOEt, 0-8% AcOEt/MeOH)