Formate → N-{2,4-Dimethyl-5-[6-(pyridin-3-yl)-1H-imidazo[1,2-b]pyrazol-1-yl]phenyl}-3-(2-hydroxypropan-2-yl)benzamide

Participants

Reaction Steps

250 mg (0.824 mmol) of the compound of Example 11A and 156 mg (0.865 mmol) of the compound of Example 92A were dissolved in 6.3 ml of DMF, and 376 mg (0.989 mmol) of HATU and 215 μl (1.24 mmol) of N,N-diisopropylethylamine were added in succession. After about 16 h of stirring at RT, the reaction mixture was diluted with about 3 ml of acetonitrile and then, in three portions, separated into its components by preparative HPLC (Method 36). After pooling and evaporation of the product fractions, the residue obtained was dissolved in a small amount of methanol and passed through a bicarbonate cartridge (from Polymerlabs, Stratospheres SPE, PL-HCO3 MP SPE, capacity 0.9 mmol) to prepare the free base from the formate salt from HPLC purification. Since the product obtained in this manner was still impure, it was purified further by MPLC (about 15 g of silica gel, mobile phase: cyclohexane/ethyl acetate/methanol 50:50:0→0:100:0→0:91:9). Evaporation of the product fractions and drying of the residue under high vacuum gave 238 mg (58% of theory, 95% pure) of the title compound.

Provenance & Evidence