Cephapirin → HCID 178475885, compound

HRID 1058487Notes

Reaction equation

HRID 1058487 reaction equation
[CH3:1][C:2]1([CH3:65])[C:21]([CH:22]=[CH:23][CH:24]=[CH:25][CH:26]=[C:27]2[N:31]([CH2:32][CH2:33][CH2:34][CH2:35][S:36]([OH:39])(=[O:38])=[O:37])[C:30]3[CH:40]=[CH:41][C:42]([CH2:44][C:45]([O:47][N:48]4[C:53](=[O:54])[CH2:52][CH2:51][C:49]4=[O:50])=[O:46])=[CH:43][C:29]=3[C:28]2([CH3:56])[CH3:55])=[N+:20]([CH2:57][CH2:58][CH2:59][CH2:60][S:61]([O-:64])(=[O:63])=[O:62])[C:4]2[CH:5]=[CH:6][C:7]([CH2:9][C:10]([O:12][N:13]3[C:18](=[O:19])[CH2:17][CH2:16][C:14]3=[O:15])=[O:11])=[CH:8][C:3]1=2.[Na+:66].[CH3:67][C:68]([O:70][CH2:71][C:72]1[CH2:91][S:90][C@@H:75]2[C@H:76]([NH:79][C:80]([CH2:82][S:83][C:84]3[CH:85]=[CH:86][N:87]=[CH:88][CH:89]=3)=[O:81])[C:77](=[O:78])[N:74]2[C:73]=1[C:92]([OH:94])=[O:93])=[O:69]>P([O-])([O-])([O-])=O.[K+].[K+].[K+]>[CH3:67][C:68]([O:70][CH2:71][C:72]1[CH2:91][S:90][C@@H:75]2[C@H:76]([NH:79][C:80]([CH2:82][S:83][C:84]3[CH:89]=[CH:88][N:87]=[CH:86][CH:85]=3)=[O:81])[C:77](=[O:78])[N:74]2[C:73]=1[C:92]([OH:94])=[O:93])=[O:69].[CH3:1][C:2]1([CH3:65])[C:21]([CH:22]=[CH:23][CH:24]=[CH:25][CH:26]=[C:27]2[N:31]([CH2:32][CH2:33][CH2:34][CH2:35][S:36]([OH:39])(=[O:37])=[O:38])[C:30]3[CH:40]=[CH:41][C:42]([CH2:44][C:45]([O:47][N:48]4[C:53](=[O:54])[CH2:52][CH2:51][C:49]4=[O:50])=[O:46])=[CH:43][C:29]=3[C:28]2([CH3:55])[CH3:56])=[N+:20]([CH2:57][CH2:58][CH2:59][CH2:60][S:61]([O-:64])(=[O:62])=[O:63])[C:4]2[CH:5]=[CH:6][C:7]([CH2:9][C:10]([O:12][N:13]3[C:14](=[O:15])[CH2:16][CH2:17][C:18]3=[O:19])=[O:11])=[CH:8][C:3]1=2.[Na+:66]

Participants

4

Reactant 2

HCID 30699Cephapirin3×HCID 178424924HCID 178424924, compound2×

Product 1

HCID 178475885HCID 178475885, compound

Reagents, catalysts & solvents 1

HCID 62657Potassium Phosphate, TribasicSolvent2×

Procedure

1 milligram of activated Cy-5® dye (Biological Detector Systems) dissolved in 0.2 ml of 20 mM potassium phosphate buffer, pH 7.2 was combined with 2 ml of a Cephapirin antibody solution (4 mg/ml Cephapirin antibody in 20 mM potassium phosphate buffer at pH 7.2). Cy-5® dye/Cephapirin solution was then stirred at room temperature for 1 hour. The resultant Cy-5®-Cephapirin conjugate was purified on a gel filtration column (Sephadex G-25), where 20 mM potassium phosphate buffer, pH 7.2, was used as both the equilibration and elution buffer.

Workup

  1. filtrationThe resultant Cy-5®-Cephapirin conjugate was purified on a gel filtration column (Sephadex G-25), where 20 mM potassium phosphate buffer, pH 7.2
  2. washthe equilibration and elution buffer

Sources & evidence

ORD record
ord-18da5d9bf7f544d6877c2cc505f82cd9
Source dataset
uspto-grants-2003_02
Patent number
US06517778B1

Reaction notes

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