HRID1061480

反应详情

EQUATION

反应方程式

REACTANTS

反应物

结构：CC(=O)N(O)CCCCCNC(=O)CCC(=O)N(O)CCCCCNC(=O)CCC(=O)N(O)CCCCCN

HCID2973

Deferoxamine

C25H48N6O8

2 次

结构：O=C1OC2(c3ccc(O)cc3Oc3cc(O)ccc32)c2ccc(N=C=S)cc21

HCID18730

Fluorescein-5-isothiocyanate

C21H11NO5S

2 次

PRODUCTS

生成物

结构：CC(=O)N(O)CCCCCNC(=O)CCC(=O)N(O)CCCCCNC(=O)CCC(=O)N(O)CCCCCN.O=C(O)c1ccccc1-c1c2ccc(=O)cc-2oc2cc(O)ccc12

HCID86583864

未命名化合物

AUXILIARIES

试剂、催化剂与溶剂

溶剂HCID5359268

Aluminum

PROCEDURE

实验过程

An aliquot of 120 mg of Desferrioxamine and 233.64 mg of fluorescein isothiocyanate (FITC) are mixed in 50 ml of 0.1 M Na carvonate pH 9.5. A molar ratio of 3:1 of FITC: Desferrioxamine is used as described in Hentz et al. (1997) Anal. Chem. 69:4994-5000. The reaction mixture is wrapped in aluminum foil to avoid light exposure and allowed to react for at least 24 hours at room temperature. To separate fluorescent-Desferrioxamine from free fluorescein and free Desferrioxamine, the mixture is electrophoresed over a P-2 column (Bio-Rad) equilibrated in saline and samples are eluted in 2 ml fractions. The fluorescein-Desferrioxamine is eluted in the void volume of the column. The first peak is collected and used for preparing fluorescein-Desferrioxamine, Desferrioxamine, and rhodamine-liposomes.

WORKUP

后处理

customto react for at least 24 hours at room temperature
customTo separate fluorescent-Desferrioxamine from free fluorescein and free Desferrioxamine
washare eluted in 2 ml fractions
washThe fluorescein-Desferrioxamine is eluted in the void volume of the column
customThe first peak is collected
customused for preparing fluorescein-Desferrioxamine, Desferrioxamine, and rhodamine-liposomes

来源与证据

来源类型: Open Reaction Database
ORD 记录: ord-d6f5b387ebd944f0835dcb70f656f92a
来源数据集: uspto-grants-2005_11
DOI: 10.6084/m9.figshare.5104873.v1
专利: US06960560B2
来源说明: uspto-grants-2005_11

反应 SMILES

[CH3:1][C:2]([N:4]([OH:39])[CH2:5][CH2:6][CH2:7][CH2:8][CH2:9][NH:10][C:11]([CH2:13][CH2:14][C:15]([N:17]([OH:38])[CH2:18][CH2:19][CH2:20][CH2:21][CH2:22][NH:23][C:24]([CH2:26][CH2:27][C:28]([N:30]([OH:37])[CH2:31][CH2:32][CH2:33][CH2:34][CH2:35][NH2:36])=[O:29])=[O:25])=[O:16])=[O:12])=[O:3].[CH:40]1[C:45](N=C=S)=[CH:44][C:43]2[C:49]([O:51][C:52]3([C:62]4[CH:63]=[CH:64][C:65]([OH:67])=[CH:66][C:61]=4[O:60][C:54]4[CH:55]=[C:56]([OH:59])[CH:57]=[CH:58][C:53]3=4)[C:42]=2[CH:41]=1)=[O:50]>[Al]>[CH:40]1[CH:45]=[CH:44][C:43]([C:49]([OH:51])=[O:50])=[C:42]([C:52]2[C:53]3[CH:58]=[CH:57][C:56]([OH:59])=[CH:55][C:54]=3[O:60][C:61]3[C:62]=2[CH:63]=[CH:64][C:65]([CH:66]=3)=[O:67])[CH:41]=1.[CH3:1][C:2]([N:4]([OH:39])[CH2:5][CH2:6][CH2:7][CH2:8][CH2:9][NH:10][C:11]([CH2:13][CH2:14][C:15]([N:17]([OH:38])[CH2:18][CH2:19][CH2:20][CH2:21][CH2:22][NH:23][C:24]([CH2:26][CH2:27][C:28]([N:30]([OH:37])[CH2:31][CH2:32][CH2:33][CH2:34][CH2:35][NH2:36])=[O:29])=[O:25])=[O:16])=[O:12])=[O:3]