7-Aminocephalosporanic acid → HCID 131712722, compound

Participants

Reaction Conditions

Reaction Steps

To a hot solution (50°-55° C.) of 6-mercapto-8-aminocarbonyl-tetrazolo[1,5-b]pyridazine (2 g; 0.010 mole) and 2.8 g of sodium bicarbonate in 90 ml of buffer phosphate (pH 6.4), 7-ACA (4.6 g; 0.017 mole) was added portionwise and the mixture was heated at 60° C. for 4 hours. After cooling at 10° C. the precipitated 7-amino-3-[(8-aminocarbonyl-6-tetrazolo[1,5-b]pyridazinyl-thiomethyl]-3-cephem-4-carboxylic acid was collected by filtration. The solid was suspended in 150 ml of Me2CO:H2O (1:2), stirred for 30 minutes and filtered again. The collected precipitate was washed with Me2CO and dried under vacuum at 60° C. to give the title compound (80%), m.p. 228°-230° C. (dec.).

Provenance & Evidence