反应详情

A solution of Nα-benzyl-Nδ-Boc-Nα-methylornithine (300 mg), diisopropylethylamine (326 μl), and anhydrous tetrahydrofuran (4 ml) was treated with PyBop (585 mg) at 0° C. for 10 min., followed by the addition of a solution of 3-phenylpropylamine (147 μl) in anhydrous tetrahydrofuran (2 ml). The resulting solution was stirred at ambient temperature for 4 hrs. The reaction was diluted with ethyl acetate and washed with water. The organic layer was dried over anhydrous sodium sulfate and concentrated in vacuo. The product was purified by chromatography over silica gel (hexane/ethyl acetate) to give Nα-benzyl-Nδ-Boc-Nα-methylornithine 3-phenylpropylamide (370 mg): 1H NMR (400 MHz, CDCl3) δ1.43 (s, 9H), 1.6-1.9 (m, 6H), 2.2 (s, 3H), 2.65 (t, J=6.2 Hz, 2H), 3.0-3.4 (m, 5H), 3.64 (s, 2H), 4.8 (s, 1H), and 7.1-7.5 (m, 10H).

1. washwashed with water
2. dry with materialThe organic layer was dried over anhydrous sodium sulfate
3. concentrationconcentrated in vacuo
4. customThe product was purified by chromatography over silica gel (hexane/ethyl acetate)