alpha-(R)-(3-(S)-((4-(benzothiazol-2-yl)piperidin-1-yl)methyl)-4-(S)-phenylpyrrolidin-1-yl)-cyclohexaneacetic acid, 4-methoxybenzyl ester → alpha-(R)-(3-(S)-((4-(Benzothiazol-2-yl)piperidin-1-yl)methyl)-4-(S)-phenylpyrrolidin-1-yl)-cyclohexaneacetic Acid

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Reaction Steps

A solution of 50 mg of α-(R)-(3-(S)-((4-(benzothiazol-2-yl)piperidin-1-yl)methyl)-4-(S)-phenylpyrrolidin-1-yl)-cyclohexaneacetic acid, 4-methoxybenzyl ester (from Step C) in 2 mL of formic acid (96%) was stirred at room temperature for 18 hours. After concentration under reduced pressure, the residue was purified by flash chromatography eluting with 10% MeOH in CH2Cl2, and then CHCl3: MeOH: NH4OH=80:15:1 to give 34 mg of the title compound as a white solid. 1H NMR (400 MHz, CD3OD): δ1.10-2.35 (m, 9H), 2.41 (br d, 2H), 2.56 (br t, 2H), 2.8-3.25 (m, 10 H), 3.4-3.75 (m, 6H), 7.25-7.45 (m, 6H), 7.48 (t, J=7.3 Hz, 1H), 7.89 (d, J=7.8 Hz, 1H), 7.93 (d, J=8.2 Hz, 1H); ESI-MS 518 (M+1); HPLC A: 2.88 min.

Provenance & Evidence