Methyl L-phenylalaninate hydrochloride → Aspartame

Participants

Reaction Steps

80 μl buffer (0.5 M Hepes/Na+; pH 7) are added to 50 mg (0.2 mmol) AC-Asp(OK)-OK and 83 mg (0.4 mmol) H-Phe-OME.HCl in a closable polypropylene vessel and the components are immediately mixed intensively with a spatula. The reaction mixture is thermostatically controlled at 40° C. on a water-bath and the reaction is then started with 20 μl buffer comprising 4 mg thermolysin preparation (Sigma P 1512).

Provenance & Evidence