HCID 178462205, compound → HCID 178462166, compound

Participants

Reaction Conditions

Reaction Steps

A mixture of 300 mg (0.554 mmol) of 3-[3-(N-{N-Boc-L-alanyl}-aminomethyl)-phenylamino]-4-(3-chloro-phenylamino)-1H-pyrazolo[3,4-d]pyrimidine (water content 0.83 %; see Example 98) and 10 ml of 4N methanolic hydrochloric acid is stirred at 20° C. for 21 hours and then concentrated by evaporation in vacuo. Recrystallization of the residue from ethanol/diethyl ether yields 4-(3chloro-phenylamino)-3-[3-(N-{L-alanyl}-aminomethyl)-phenylamino]-1H-pyrazolo[3,4-d]pyrimidine.1.7 hydrochloride having a water content of 6.83%; m.p. 222-225° C.; ESI-MS: (M+H)+=437; [a]D20=+1.6±1.1° (c=0.893%, methanol).

Provenance & Evidence