Phosphate → Ribavirin

Participants

Reaction Steps

The whole amount of washed cells (corresponding to 5 ml of the culture solution) of microorganisms shown in Table 2 prepared by culturing them in a manner similar to Example 1 was added to 5 ml of 0.3 M phosphate buffer (adjusted final pH to 7.0) containing 50 mM of orotidine or orotidic acid and 50 mM of 1,2,4-triazole-3-carboxamide followed by reacting at 60° C. for 5 hours. The concentration of ribavirin produced in each reaction solution was measured by a known method using high performance liquid chromatography. The results are shown in Table 2.

Provenance & Evidence