Phosphate → Ribavirin

HRID 1073052Notes

Reaction equation

HRID 1073052 reaction equation
P([O-])([O-])([O-])=O.[C@@H:6]1(N2C(C(O)=O)=CC(=O)NC2=O)[O:14][C@H:11]([CH2:12][OH:13])[C@@H:9]([OH:10])[C@H:7]1[OH:8].[NH:26]1[CH:30]=[N:29][C:28]([C:31]([NH2:33])=[O:32])=[N:27]1>>[CH:30]1[N:26]([C@@H:6]2[O:14][C@H:11]([CH2:12][OH:13])[C@@H:9]([OH:10])[C@H:7]2[OH:8])[N:27]=[C:28]([C:31]([NH2:33])=[O:32])[N:29]=1

Participants

4

Reactant 3

HCID 1061PhosphateHCID 927516-Hydroxy-2-oxo-3-pentofuranosyl-2,3-dihydropyrimidine-4-carboxylic acidHCID 178513825HCID 178513825, compound

Product 1

HCID 37542Ribavirin

Procedure

prepared by culturing the same in a manner similar to Example 1 was added to 5 ml of 0.3 M phosphate buffer (adjusted final pH to 7.0) containing 50 mM of orotidine or orotidic acid and 50 mM of 1,2,4-triazole-3-carboxamide. The whole amount of washed cells (corresponding to 5 ml of the culture solution) of microorganisms shown in Table 5 prepared by culturing them in a manner similar to Example 1 was added to each reaction solution to prepare 28 kinds of reaction solutions shown in Table 5. Each reaction solution followed was reacted at 60° C. for 5 hours. The concentration of ribavirin produced was measured in a manner similar to Example 2. The results are shown in Table 5.

Workup

  1. customprepared
  2. customprepared
  3. additionwas added to each reaction solution
  4. customto prepare
  5. custom28 kinds of reaction solutions
  6. customEach reaction solution followed was reacted at 60° C. for 5 hours

Sources & evidence

ORD record
ord-03bc969abb4740b59838cb944562199a
Source dataset
uspto-grants-1990_11
Patent number
US04968606

Reaction notes

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