HCID 178474910, compound → HCID 178474911, compound

Participants

Reaction Steps

To a solution of 467 mg of sodium 7β-[2-(2-aminothiazol-4-yl)-(Z)-2-ethoxyiminoacetamido]-3-hydroxymethyl -3-cephem-4-carboxylate and 374 mg of 1-(2-guanidinoethyl) -5-mercapto-1H-tetrazole in 10 ml of DMF was added 500 mg of ethyl o-phenylenephosphate at -20° C. under stirring. The reaction mixture was stirred at -20° C. to 0° C. for 60 minutes, and was subjected to a column chromatography on silica gel (100 g), being washed with acetonitrile and eluted with a (5:1) mixture of acetonitrile and water. The eluate was concentrated under reduced pressure, and then subjected to a column chromatography on XAD-II (150 ml), being washed with water and eluted with 20% aqueous ethanol. The eluate was concentrated under reduced pressure and then lyophilized to give 333 mg of the title compound as a colorless powder.

Provenance & Evidence