HCID 23662671, compound → HCID 15159513, compound

Participants

Reaction Conditions

Reaction Steps

To a solution of sodium 7β-[2-(5-amino-1,2,4-thiadiazol-3-yl)-(Z)-2-ethoxyiminoacetamido]-3-hydroxymethyl-3 -cephem-4-carboxylate (360 mg) and 1-(2-amidinomethylthioethyl)-5-mercapto-1H-tetrazole (262 mg) in 8 ml of DMF was added 1.0 g of ethyl o-phenylenephophate under stirring at -20° C. After stirring under ice-cooling for 1.5 hours, the reaction mixture was subjected to a column chromatography (silica gel, 100 g), being washed with acetonitrile and eluted with acetonitrile/water (4:1). The eluate was concentrated under reduced pressure and then subjected to a column chromatography (XAD-II, 150 ml), being washed with water and eluted with 20% aqueous ethanol. The eluate was concentrated under reduced pressure and lyophilized to give 159 mg of the title compound as a colorless powder.

Provenance & Evidence