Sodium Hydroxide → 6-Aminopenicillanic acid

Participants

Reaction Steps

To a slurry of 46 g of the wet immobilized cells of Example 6 in 500 ml water at pH 8.0 and 37°C is added 5 g of potassium penicillin G. The pH of the slurry is maintained at 8.0 by the monitored addition of 1N NaOH, and the hydrolysis is complete after about 3 hours. The immobilized cells are filtered, washed with water and stored as a wet cake until further use. The filtrate is adjusted to pH 2 with 2N HCl and extracted with ethyl acetate. The resulting aqueous layer is adjusted to pH 4.3 with 2N NaOH, concentrated and cooled to give crystalline 6-amino-penicillanic acid.

Provenance & Evidence