Sodium Hydroxide → 6-Aminopenicillanic acid

Participants

Reaction Steps

To a slurry of 12 g of the wet treated immobilized cell preparation of Example 10 in 100 ml of water at pH 8.0 and 37°C is added 1 g of potassium penicillin G. The pH of the reaction is maintained at 8.0 by the monitored addition of 1N NaOH and the reaction is complete after about 3 hours. The immobilized cell material is filtered, washed with water and stored as a wet cake until further use. The filtrate is adjusted to pH 2 with 2N HCl and extracted with ethyl acetate. The resulting aqueous layer is adjusted to pH 4.3 with 2N NaOH, concentrated and cooled to give crystalline 6-APA.

Provenance & Evidence