Ammonium Hydroxide → L-Phenylalanine

Participants

Reaction Conditions

Reaction Steps

A suspension of 14.74 g of wet immobilized cells of Example 14 in 30 ml of water containing 2.25 g of trans-cinnamic acid, 652 mg of ammonium chloride and 3.3 ml of concentrated ammonium hydroxide was adjusted to pH 9.5. The mixture was shaken at 37°C for 18 hours giving about 90% yield of L-phenylalanine, based on recovered cinnamic acid. The reaction solids were filtered and washed with water to give 14 g of wet immobilized cells containing 77% of their original phenylalanine ammonia-lyase activity. The L-phenylalanine was isolated from the filtrate using standard procedures.

Provenance & Evidence