Trifluoromethanesulfonic acid → Phenylalanine amide

Participants

Reaction Steps

Bz-Phe-2,4-dimethoxybenzylamide (4.2 mg) was dissolved in trifluoroacetic acid (1.0 ml) containing 10% (v/v) trifluoromethane sulfonic acid (TFMSA) and the reaction mixture was kept in the dark for 1 h at 20° C. TFA/TFMSA was removed by blowing with N2 and the residue was suspended in 95% (v/v) CH3CN (1.0 ml). The reaction mixture was analyzed by HPLC system-7 and the generated Bz-Phe-NH2 (>95%) was determined quantitatively using a Bz-Phe-NH2 standard solution (Bz-Phe-NH2 was purchased from Bachem, Switzerland).

Provenance & Evidence