Hydrochloric Acid → Batzelline A

Participants

Reaction Steps

A solution of 20 mg of isobatzelline A in 2 ml of HOAc and 1 ml of dioxane is allowed to react with 20 mg of NaNO2 in 5 ml of H2O at 0° C. for 2 hours. After addition of 3 ml of 1N HCl, the solution was stirred at room temperature overnight, and extracted with 10 ml CHCl3 /MeOH (1:1) twice. The concentrated extract was further fractionated on a Superco LC-NH2 column (50 ml×3 ml) with 1% MeOH/CHCl3, and subsequently purified on a HPLC LiChrosorb-NH2 column (3% MeOH/CHCl3) to give 4 mg of batzelline A.

Provenance & Evidence