反应详情

温度

20 °C

A mixture of 146 mg of (6R,7R)-7-[(Z)-2-(2-amino-4-thiazolyl)-2-[(2-iodoethoxy)imino]acetamido]-3-[[[2-(hydroxymethyl)-5-methyl-s-triazolo[1,5-a]pyrimidin-7-yl]thio]methyl]-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid, 75 mg of 2.2-diphenyl-1.3-benzodioxol-5-sulphinic acid lithium salt and 24 mg of sodium hydrogen carbonate in 0.5 ml of N,N-dimethylformamide was stirred at 20° C. for 24 hours. The reaction mixture was treated with 5 ml of water and the pH of the mixture was lowered to 3 by the addition of 3N hydrochloric acid. The resulting precipitate was filtered off under suction, washed with a small amount of water and then dissolved in 4 ml of water with the addition of small amount of 2N sodium hydroxide solution. This solution, of pH 7.5, was applied to a MCI gel column (CHP2OP) conditioned with 1% aqueous acetic acid and chromatographed according to the procedure described in Example 16. The fraction eluted with a 1:1 (v/v) water/acetonitrile mixture was concentrated in a vacuum and lyophilized. The lyophilizate was dissolved in 90% aqueous trifluoroacetic acid and the solution was stirred at 20° C. for 20 minutes. The solvents were removed in a vacuum and the residue was partitioned between ethyl acetate and 2% sodium hydrogen carbonate solution. The aqueous phase was chromatographed according to the procedure described in Example 16. After concentration and lyophilization of the pure fractions, there was obtained (6R,7R)-7-[(Z)-2-(2-amino-4-thiazolyl)-2-[[2-[(3.4-dihydroxyphenyl)sulphonyl]ethoxy]imino]acetamido]-3-[[[2-(hydroxymethyl)-5-methyl-s-triazolo[1,5-a]pyrimidin-7-yl]thio]methyl]-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid as a white powder.

1. filtrationThe resulting precipitate was filtered off under suction
2. washwashed with a small amount of water
3. dissolutiondissolved in 4 ml of water with the addition of small amount of 2N sodium hydroxide solution
4. customchromatographed
5. washThe fraction eluted with a 1:1 (v/v) water/acetonitrile mixture
6. concentrationwas concentrated in a vacuum
7. dissolutionThe lyophilizate was dissolved in 90% aqueous trifluoroacetic acid
8. stirringthe solution was stirred at 20° C. for 20 minutes
9. customThe solvents were removed in a vacuum
10. customthe residue was partitioned between ethyl acetate and 2% sodium hydrogen carbonate solution
11. customThe aqueous phase was chromatographed
12. concentrationAfter concentration and lyophilization of the pure fractions