Acetic Acid → HCID 178475886, compound

Participants

Reaction Steps

To a mixture of 0.309 g of 7-[2-(2-imino-4-thiazolin-4-yl)-2-hydroxyimino-acetamido]-3-acetoxymethyl-3-cephem-4-carboxylic acid hydrochloride (syn-isomer) and 1 ml of acetic acid there was added a sufficient amount of 1N-hydrochloric acid to completely dissolve the former. This solution was subjected to column chromatography on polystyrene resin (Amberlite XAD-2, Rohm & Haas Co.), development being carried out with water and 20% ethanol in the order mentioned. The fractions containing the desired product were pooled, concentrated and lyophilized. By the above procedure the captioned compound was obtained.

Provenance & Evidence