Triethyl Phosphate → HCID 15160070, compound

Participants

Reaction Steps

A solution of 560 mg of 3-β-D deoxyribofuranosyl-2,7-dioxopyrido[2,3-d]pyrimidine (a) in 5 ml of triethylphosphate was cooled to 0° C. and 613 mg of phosphorus oxychloride was added thereto. After the mixture was made to undergo a reaction for 6 hours, 1 g of ice was added to effect a hydrolysis. The reaction mixture was concentrated at reduced pressure and the resulting residue was dissolved in 1 ml of distilled water. The purification of the product was carried out by column chromatography on DEAE-Sephadex A-25 (HCO3 -form) by use of a linear gradient of triethylammonium bicarbonate to obtain 575 mg of 3-(5'-O-phosphoryl-β-D-2'-deoxyribofuranosyl)-2,7-dioxopyrido[2,3-d]pyrimidine (b).

Provenance & Evidence