反应详情

A solution of 3-(fluoren-9-yl)propionic acid (2.4 g, 10 mmol) in thionyl chloride was refluxed for two hours. The reaction mixture was cooled down and the solvent was removed in vacuum. A solution of the resulting 3-(fluoren-9-yl)propionic acid chloride was dissolved in dioxane (20 mL) and added into a solution of L-tert-leucine (4 g, 30.5 mmol) in 10% aqueous sodium carbonate (40 mL). The reaction mixture was monitored by quenching an aliquot with methanol than monitor the resulting methyl ester by TLC (10% MeOH in chloroform). After an 1.5 hr water was added and most of the dioxane was removed in vacuum. The aqueous layer was washed with diethyl ether (×3) then was acidified to pH 1 with 10% HCl. The resulting precipitate was purified by a short path chromatography (silica, 5% MeOH in chloroform) to yield 1.5 g (42.1%), mp 79°-83° C.

1. temperatureThe reaction mixture was cooled down
2. customthe solvent was removed in vacuum
3. dissolutionA solution of the resulting 3-(fluoren-9-yl)propionic acid chloride was dissolved in dioxane (20 mL)
4. customby quenching an aliquot with methanol than monitor the resulting methyl ester by TLC (10% MeOH in chloroform)
5. additionAfter an 1.5 hr water was added and most of the dioxane
6. customwas removed in vacuum
7. washThe aqueous layer was washed with diethyl ether (×3)
8. customThe resulting precipitate was purified by a short path chromatography (silica, 5% MeOH in chloroform)
9. customto yield 1.5 g (42.1%), mp 79°-83° C.