反应详情

The whole mixture was incubated at 37° C. for 90 minutes, then the reaction was terminated by adding 1 mM of 15%-KOH (a 95% ethanol solution), next 2 mg of cholesterol was added thereto, saponified at 75° C. for 1 hour, and cholesterol was extracted with petroleum ether, the extract was concentrated to dryness. To the residue thus obtained was added with 2.5 ml of a mixture of acetone-diethyl ether (1:1) and 2.5 ml of 1%-digitonin (a 50% ethanol solution), then this solution was allowed to stand for 1 hour in cooling condition by using ice-water to precipitate cholesterol. The precipitate was washed with acetone, a mixture of acetonediethyl ether (1:1) and diethyl ether in this order, then the radioactivity of the residue was measured by using a liquid scintillation counter.

1. customthe reaction was terminated
2. additionby adding 1 mM of 15%-KOH (a 95% ethanol solution), next 2 mg of cholesterol
3. additionwas added
4. waitsaponified at 75° C. for 1 hour
5. extractioncholesterol was extracted with petroleum ether
6. concentrationthe extract was concentrated to dryness
7. customTo the residue thus obtained
8. waitto stand for 1 hour
9. temperaturein cooling condition
10. customto precipitate cholesterol
11. washThe precipitate was washed with acetone
12. additiona mixture of acetonediethyl ether (1:1) and diethyl ether in this order