Raffinose → Npc283221

HRID 1099537Notes

Reaction equation

HRID 1099537 reaction equation
C(O)(=O)CCCCCCC/C=C\CCCCCCCC.C(O)[C@H]1O[C@H]([O:28][CH2:29][C@H:30]2[O:35][C@H:34]([O:36][C@:37]3([CH2:46][OH:47])[O:41][C@H:40]([CH2:42][OH:43])[C@@H:39]([OH:44])[C@@H:38]3[OH:45])[C@H:33]([OH:48])[C@@H:32]([OH:49])[C@@H:31]2[OH:50])[C@H](O)[C@@H](O)[C@H]1O.C(O)[C@H]1O[C@H](OC[C@H]2O[C@H](OC[C@H]3O[C@H](O[C@]4(CO)O[C@H](CO)[C@@H](O)[C@@H]4O)[C@H](O)[C@@H](O)[C@@H]3O)[C@H](O)[C@@H](O)[C@H]2O)[C@H](O)[C@@H](O)[C@H]1O>>[CH2:29]([OH:28])[C@H:30]1[O:35][C@H:34]([O:36][CH:37]2[C@@H:46]([OH:47])[C@H:42]([OH:43])[CH:40]([OH:41])[C@H:39]([OH:44])[C@@H:38]2[OH:45])[C@H:33]([OH:48])[C@@H:32]([OH:49])[C@H:31]1[OH:50]

Participants

4

Reactant 3

HCID 439242RaffinoseHCID 439531StachyoseHCID 445639Oleic Acid2×

Product 1

HCID 11727586Npc283221

Procedure

T2 plants of events EAFS 4818.1.5 and EAFS 4871.6.10 that were homozygous for an YL DGAT1/YL DGAT2 event (Example 6) and a PGM/RFO ko event (Example 31), respectively were crossed. Resulting F1 seed were analyzed by GC and TLC as described above to monitor presence of the DGAT trait (elevated oleic acid) and PGM/RFO ko trait (reduced raffinose and stachyose). A total of 23 F1 seed with elevated oleic acid content and reduced raffinosaccharide levels were planted, resulting plants were grown to maturity. For every F1 plant a total of 48 F2 seed were subjected to the following analysis sequence. Seed oil content was measured non-destructively by NMR (Example 4). Seed were chipped and the resulting seed chip was extracted with heptane. Heptane extracts were subjected to GC analysis to determine the presence of the DGAT transgene (indicated by the altered fatty acid profile of the oil). The heptane-extracted residue was subjected to TLC analysis of raffinose and stachyose content (Example 29) to determine presence or absence of the EAFS 4871.6.10 transgene. In this manner 1104 seed were genotyped and the effect of transgene genotype on oil content was determined.

Workup

  1. customresulting plants
  2. extractionthe resulting seed chip was extracted with heptane
  3. extractionThe heptane-extracted residue

Sources & evidence

ORD record
ord-70cd9b1d301b4a64a0bce0cac9fb027c
Source dataset
uspto-grants-2012_03
Patent number
US08143476B2

Reaction notes

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