Sodium Bicarbonate → HCID 53832674, compound

Participants

Reaction Steps

A solution of 211 mg of sodium bicarbonate in 2.6 ml of water is added to a solution of 100 mg of 19,21-dihydroxypregna-4,6-diene-3,20-dione diacetate in 6.5 ml of methanol and the mixture is heated under reflux for 3 hours and concentrated in vacuo. A solution of the residue in methylene chloride is washed with a 15% aqueous sodium chloride solution, dried with sodium sulphate and concentrated in vacuo. The residue is taken up in a mixture of hexane and ethyl acetate (1:1) and chromatographed over silica gel. After evaporating off the solvent, the product is dissolved and allowed to crystallise from methylene chloride/diethyl ether/diisopropyl ether, yielding 19,21-dihydroxypregna-4,6-diene-3,20-dione, melting point: 161°-164°.

Provenance & Evidence