HuagongsheHuagongshe Chemistry Print Report · For research reference only
Adenosine → Purine
Participants
| Role | Name | HCID | Charge |
|---|---|---|---|
| Reactant | Adenosine | HCID 60961 | — |
| Reactant | Guanosine | HCID 135398635 | — |
| Reactant | (2R,3S,4R,5R)-2-(hydroxymethyl)-5-(6-hydroxypurin-9-yl)oxolane-3,4-diol | HCID 178521066 | — |
| Product | Purine | HCID 1044 | — |
Reaction Steps
The optimum pH was determined by high-performance liquid chromatography with adenosine, guanosine and inosine substrates. The reaction solution (200 μl) contained 0.5 mM of adenosine or inosine or 0.25 mM of guanosine as the substrate, 50 mM of the buffer solutions described below and a sufficient amount of enzyme, and the reaction was conducted at 50° C. for 30 minutes. The results are shown in FIGS. 2, 3 and 4.
Provenance & Evidence
| DOI | 10.6084/m9.figshare.5104873.v1 |
|---|---|
| Patent number | US06066484 |
| Source dataset | uspto-grants-2000_05 |
| ORD ID | ord-ef898500902442a5a0caeb6a55f42f2c |
| Source note | uspto-grants-2000_05 |