Benzene → 16846-24-5

Participants

Reaction Steps

After developing the plate in a developer composed of n-hexane:benzene:methanol:ethyl acetate=30:10:25:8:20, and product spots were tested by color development with sulfuric acid to try to detect 3-acylated leucomycin A1. As a result, it was found that from among about 200 transformed strains, Streptomyces lividans 53A strain (hereafter, referred to as "strain 53A") produced leucomycin A3, i.e., 3-O-acetyl leucomycin A1. The leucomycin produced by the strain 53A was identified to be leucomycin A3 from results of analyses of TLC, HPLC, UV spectrum, NMR spectrum, antibacterial spectrum and the like.

Provenance & Evidence