Indomethacin → (3H)Ltd4

Participants

Reaction Steps

Antagonism for LTD4 was determined in isolated male guinea-pig (300-400 g) trachea prepared as spiral strip. Tracheal preparations were suspended under 1 g tension in 10 ml organ baths containing 5 μM of indomethacin and they were incubated for 1 hr prior to use. Contractile responses to LTD4 (2×10-8 g/ml) were obtained after the maximal response to histamine (10- 4 M). Test compounds dissolved in 100% dimethyl sulfoxide were added to the organ baths (final concentration of 10-6 g/ml or 10-7 g/ml) 30 min prior to LTD4 addition, and then contractile responses to LTD4 were compared with those of control which was obtained from a paired trachea in the absence of test compounds. LTD4 -induced contractions were expressed as a percentage of the maximal response to histamine. The antagonism was determined as follows:

Provenance & Evidence