反应详情

The resulting crude 7β-phenoxyacetamido-3-hydroxy-ceph-3-em-4-carboxylic acid diphenylmethyl ester is dissolved in methanol and a solution of diazomethane in ether is added at 0° C. After 10 minutes, the solution is carefully concentrated and the residue is dried under a high vacuum. The residue is purified by thick layer chromatography (toluene/ethyl acetate, 3:1, silica gel). After eluting the silica gel of the zone at Rf=0.17 with ethyl acetate, and concentrating the solution on a rotary evaporator, 7β-phenoxyacetamido-3-methoxy-ceph-3-em-4-carboxylic acid diphenylmethyl ester is obtained; melting point 120° C. (from ether).

1. concentrationthe solution is carefully concentrated
2. customthe residue is dried under a high vacuum
3. customThe residue is purified by thick layer chromatography (toluene/ethyl acetate, 3:1, silica gel)
4. washAfter eluting the silica gel of the zone at Rf=0.17 with ethyl acetate
5. concentrationconcentrating the solution
6. customon a rotary evaporator