Glutaraldehyde → Glucose galactose

HRID 1155750Notes

Reaction equation

HRID 1155750 reaction equation
[OH:1][CH:2]1[O:21][C@H:20]([CH2:22][OH:23])[C@@H:7]([O:8][C@@H]2O[C@H](CO)[C@H](O)[C@H](O)[C@H]2O)[C@H:5]([OH:6])[C@H:3]1[OH:4].C(=O)CCCC=O>P([O-])([O-])([O-])=O>[O:1]=[CH:2][C@@H:3]([C@H:5]([C@@H:7]([C@@H:20]([CH2:22][OH:23])[OH:21])[OH:8])[OH:6])[OH:4].[O:1]=[CH:2][C@@H:3]([C@H:5]([C@H:7]([C@@H:20]([CH2:22][OH:23])[OH:21])[OH:8])[OH:6])[OH:4]

Participants

4

Reactant 2

HCID 3485GlutaraldehydeHCID 440995(+)-Lactose5×

Product 1

HCID 86738397Glucose galactose

Reagents, catalysts & solvents 1

HCID 1061PhosphateSolvent

Conditions

Temperature
60 °C

Procedure

Pure lactose, demineralised whey and skimmed milk are used as lactose sources for the glutaraldehyde-treated DEAE-cellulose immobilised cells and the results obtained are given in the accompanying diagram, FIG. 10. A 5% solution of lactose in 50 mM phosphate buffer, pH 7.0 (line 1); a solution of demineralised, ultrafiltered whey containing 48.95 mg per ml of lactose and at pH 6.55 (line 2); and skim milk containing 48.50 mg per ml of lactose and at pH 6.86 (line 3), are eluted through a column maintained at 60° C. containing glutaraldehyde-treated DEAE-cellulose immobilised cells (bed volume 41 ml). The flow rates are 28.3, 29.5 and 29.5 ml per hour respectively for the three substrates. The amount of glucose/galactose formed in samples of eluate is determined and the percentage hydrolysis calculated.

Workup

  1. customthe results obtained
  2. washat pH 6.86 (line 3), are eluted through a column
  3. additioncontaining glutaraldehyde-
  4. additiontreated DEAE-cellulose immobilised cells (bed volume 41 ml)

Sources & evidence

ORD record
ord-b63aab503e294646b0ef3c865a2b6056
Source dataset
uspto-grants-1982_06
Patent number
US04332895

Reaction notes

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