Participants
| Role | Name | HCID | Charge |
|---|
| Reactant | DL-Serine | HCID 617 | — |
| Reactant | Indole | HCID 798 | — |
| Catalyst | Pyridoxal 5'-phosphate | HCID 1051 | — |
| Catalyst | Sodium Sulfite | HCID 24437 | — |
| Solvent | X 100 | HCID 19153 | — |
| Product | L-Tryptophan | HCID 6305 | 81.7% |
Reaction Steps
In 50 g of Triton X-100 was dissolved 20 g of indole, and 36 g of DL-serine, 1 g of Na2SO3, 100 mg of pyridoxal phosphate and the above-mentioned two kinds of cells collected by centrifugal separation were added to the solution so that the entire volume was 1 l. The reaction liquid was shaken at a temperature of 30° C. and pH of 8.5 for 72 hours to effect reaction. After the reaction, 28.5 g of L-tryptophan was formed and accumulated in the reaction liquid (the yield was 41% based on serine).
Provenance & Evidence
| DOI | 10.6084/m9.figshare.5104873.v1 |
|---|
| Patent number | US04335209 |
|---|
| Source dataset | uspto-grants-1982_06 |
|---|
| ORD ID | ord-d56553f21b0c44bb8d3d579960d98a3f |
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| Source note | uspto-grants-1982_06 |
|---|
