Potassium Chloride → Phosphate Buffered Saline

HRID 1156394Notes

Reaction equation

HRID 1156394 reaction equation
[Na+:1].[Cl-:2].C1N(CCS(O)(=O)=O)CCOC1.[Cl-].[K+:16].[OH:17][P:18]([O-:21])([OH:20])=[O:19].[K+]>>[OH:19][P:18]([O-:21])([OH:20])=[O:17].[OH:19][P:18]([O-:21])([O-:20])=[O:17].[Na+:1].[Na+:1].[Na+:1].[Cl-:2].[Cl-:2].[K+:16].[K+:16]

Participants

5

Reactant 4

HCID 4873Potassium ChlorideHCID 5234Sodium Chloride2×HCID 781654-Morpholineethanesulfonic acidHCID 516951Monobasic potassium phosphate

Product 1

HCID 24978514Phosphate Buffered Saline

Conditions

Temperature
124 °C

Procedure

Polyacrylamide Bio-Carriers (Bio-Rad Laboratories, Richmond, Calif.) with a swollen surface area of 16.5 m2 or the equivalent of 239 roller bottles (each having 690 cm2 available surface) were sterilized by autoclaving in aqueous buffer solution at pH 5.0 consisting of 0.05 M NaCl and 0.05 M 2(N-Morpholino)ethane sulfonic acid (MES). During autoclaving, the depth of the settled beads was less than two inches (<5.18 cm metric) and the temperature was maintained at 124° C. for about 40 to 60 minutes. After cooling, the beads were asceptically washed twice with phosphate buffered saline (PBS) and once with the aforesaid serum-containing medium. The PBS was prepared by dissolving 80 grams NaCl, 2 grams KCl, 2 grams KH2PO4 and 21.6 grams Na2HPO4.7H2O in 10 liters distilled water, followed by autoclaving.

Workup

  1. temperatureAfter cooling
  2. washthe beads were asceptically washed twice with phosphate buffered saline (PBS) and once with the aforesaid
  3. additionserum-containing medium
  4. distillationdistilled water

Sources & evidence

ORD record
ord-150f2ccf599941558c39ab48656cb665
Source dataset
uspto-grants-1982_06
Patent number
US04335215

Reaction notes

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