Ethylenediaminetetraacetic Acid → Ethylenediaminetetraacetic acid disodium salt
Reaction equation
CCCCCCCCCCCCOS([O-])(=O)=O.[Na+:18].[CH2:19]([N:30]([CH2:35][C:36]([OH:38])=[O:37])[CH2:31][C:32]([OH:34])=[O:33])[CH2:20][N:21]([CH2:26][C:27]([OH:29])=[O:28])[CH2:22][C:23]([OH:25])=[O:24]>>[Na+:18].[Na+:18].[CH2:20]([N:21]([CH2:26][C:27]([OH:29])=[O:28])[CH2:22][C:23]([OH:25])=[O:24])[CH2:19][N:30]([CH2:35][C:36]([O-:38])=[O:37])[CH2:31][C:32]([O-:34])=[O:33]Participants
Conditions
- Temperature
- -20 °C
Procedure
Samples were prepared for reducing SDS-PAGE by adding 110 μl of sample to 10 μl sample Buffer (2×), 2.5 μl reducing agent (10×) and 2 μl of 0.1M EDTA (to achieve final concentration of 10 mM). The high molecular weight (HMW) marker was prepared by adding 10 μl of concentrated stock to 80 μl reducing agent (10×), 310 μl WFI and 400 μl sample buffer (2×). The diluted HMW standard was then heated at 95° C. for 5 minutes before aliquoting and storage at −20° C. for use in subsequent gels. Samples (15 μl) containing collagenases were run directly (i.e. with no prior heat treatment) on 8% Tris-Glycine gels using Tris-Glycine running buffer at 130 V for ˜1 hour 50 mins. After electrophoresis, the gels were stained with colloidal blue stain reagent as per the manufacturer's instructions.
Workup
- customSamples were prepared
- additionby adding 110 μl of sample to 10 μl sample Buffer (2×), 2.5 μl
- customThe high molecular weight (HMW) marker was prepared
- additionby adding 10 μl of concentrated stock to 80 μl
- additionSamples (15 μl) containing collagenases
- customfor ˜1 hour
- custom50 mins
Sources & evidence
- ORD record
- ord-9a18576e587a4314b4115078c0882a27
- Source dataset
- uspto-grants-2010_10
- Patent number
- US07811560B2
Reaction notes
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